Mushroom Spawn Technology- Training Course

Mastering Mushroom Spawn: From Lab Bench to Commercial Scale” is an in-depth, step-by-step program dedicated exclusively to the art and science of spawn production. You’ll move from basic tissue culture to large-scale spawn manufacturing, gaining the technical know-how to produce robust, contamination-free spawn for all your favourite gourmet and medicinal mushrooms. Course Duration is for one year from date of enrolment. Course can be accessed via BM Mushroom Mobile app or any Web Browser. What You’ll Learn 1. Fundamentals of Sterile Technique Lab Setup & Hygiene: Design a mini-lab or home workspace; maintain aseptic conditions with pressure cookers, laminar flow hoods, and proper disinfectants. Media Preparation: Formulate and sterilize agar, grain, sawdust, and liquid media with precise moisture and pH control. 2. Tissue Culture & Spore Work Tissue Culture Technology: Excise clean mushroom tissue; initiate pure mycelium cultures on agar plates. Spore Technology: Collect and germinate spores; develop stable monokaryotic strains for breeding and experimentation. 3. Solid & Liquid Cultures Solid Culture: Scale from petri dishes to test tubes and jars; achieve rapid, uniform colonization. Liquid Culture: Prepare nutrient broths; use magnetic stirring and incubators to produce high-density mycelial slurries. 4. Mother Spawn & Master Culture Mother Spawn: Transition from lab cultures to bulk grain; establish a reliable mother spawn bank for repeat use. Master Culture Maintenance: Store and rejuvenate cultures for long-term viability using cryopreservation and slant techniques. 5. Commercial Spawn Types Learn specialized techniques for: Grain Spawn (wheat, sorghum, millet) Sawdust Spawn (hardwood and softwood blends) Wood Chip & Beechwood Spawn Liquid Mycelial Slurry for rapid inoculations 6. Variety-Specific Protocols Advanced spawn recipes and conditions for: Oyster (Pleurotus spp.) Button (Agaricus bisporus) Milky (Calocybe indica) Much more..